Investigating the molecular basis of oligomerisation specificity using Essay

Investigating the molecular basis of oligomerisation specificity using coiled coil peptides - Essay Example The studies have identified that there is unexpected and general correlation between trigger sequences (Indispensable elements for coiled coil formation) and coiled coil oligomerization state specificity .Through the use yeast archytype coiled-coil domain transcriptional factor activator GCN4 as a model system, it was found that oligomerization state determinants that are trimer specific and well established ,switched the topology of the peptide to a trimer from a dimer, only after being inserted into the trigger sequence. The results were confirmed with 2 other coiled coil dimmers that is not related (cortexillin-1 and ATF1).It is further indicated that multiple topology determinants have the capacity of co-existing in the same trigger sequence, and this reveals a delicate balance of the resultant oligomerization state through forces that are position dependent (Steinmetz et al., 2007). Due to the simplicity of this leucine zipper, the alpha helical coiled coil has been traditionall y used in a broad number of studies that have targeted the key principles of protein stability, oligomerization and folding (Steinmetz et al., 2007).In this case, the coiled coils currently are exploited as multipurpose tool in various applications seen to be increasing steadily as well as ranging from basic research to medicine. It is well known that what is referred to as trigger sequence plays a critical role in controlling coiled –coil formation. The distinct feature of several trigger sequences is that they have a capacity of folding into several reasonable stable monomeric helices before the formation of the coiled coil structure (Kammerer et al.1998) Recently, the NMR structures of peptide spanning the GCN4 trigger sequence was solved and indicated that the structure is stabilized by electrostatic interactions and a network of hydrogen bonds (Steinmetz et al., 2007) The trigger

Analysis argument Essay Example | Topics and Well Written Essays - 750 words

Analysis argument - Essay Example These are just two examples; there are countless similar occasions in the article where statements have been made without evidence. Anderson’s article is more of an essay than an argument since throughout the article, he has just expressed personal opinions without providing evidence for them to enhance the credibility of his argument. Anderson has basically used the article as a means to express his personal opinions. Basically the article is a reflection on the author’s personal experiences that have led him to developing certain views that he has shared in an organized way. This makes the article informative rather than argumentative. Although the article’s credibility is little because of lack of references, yet the views Anderson has shared are sensible and seem to be coming from a learned and experienced author. I particularly agree to the fact that playing video games may be one of the risk factors that play a role in making the youth criminals, yet it is too subtle and ineffective a factor to have such a large negative effect on the psychology of the children as to make them killers. One factor that is particularly out of place in the article is that although Anderson has cited no study, yet he has gone to such lengths as comparing different studies and the underlying factors as sample sizes, as he says, â€Å"Why have different researchers found different results? Well, part of the problem is that many studies have used a sample size that is much too small to produce consistent results† (Anderson cited in Ramage, Bean, and Johnson 447). Greenberg has started his argument with a strong point saying that Yee had Sen. Leland Yee did not have robust grounds to impose restriction on children’s access to violent video games since it was essentially an outcome of the ban on substance abuse which in no way compares to the video games. I find Greenberg’s argument very convincing that violent books and movies do not prov ide the audiences with a room to respond whereas violent games do, but the same power of interactivity that the video games provide the audiences could be held as a strong reason why they should be banned. Although Greenberg’s example was good, but in effect, it counteracted his own argument that was in favor of expression of violence in video games, though the examples Greenberg has given regarding how video games enhance the critical analysis and decision-making skills of the audiences are entirely convincing. These examples also indicate the potential ways in which violent games serve as mediums of free expression for the players, thus providing Greenberg with a firm basis for his argument. One thing that makes Greenberg’s article superior to Anderson’s is that Greenberg has not indicated studies anywhere and accordingly he did not need to cite them, whereas Anderson’s article was mostly based on the results of past studies without mentioning any in pa rticular. Greenberg’s examples in support of his argument were such that they did not require much citation or referencing, and the examples were plentiful as well. When the examples did require referencing, they were included. For example, when Greenberg discussed the Supreme Court’s ruling that school boards are not allowed to ban books in the libraries of the schools even if they are accessible outside